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Title: The role of the heat shock proteins (HSP70 and sHSP) in the thermotolerance of freshwater amphipods from contrasting habitats
Author(s): Shatilina Z.M., H.W.Riss, M.V.Protopopova, M.Trippe, E.I.Meyer, V.V.Pavlichenko, D.S.Bedulina, D.V. Axenov-Gribanov, M.A. Timofeyev
Annotation: The aim of this study was to clarify the significance of HSP70 and sHSP for thermotolerance in freshwater amphipods. We compared four amphipod species from different freshwater habitats and biogeographical regions (CentralEurope vs. LakeBaikal). Test individuals were exposed to thermal stress generated by a water temperature of 25C. The thermotolerance of the species,determined by median lethal time (LT50), followed in decreasing order by Gmelinoides fasciatus, Echinogammarus berilloni, Gammarus pulex, Eulimnogammarus verrucosus. HSP70 and sHSP base level concentrations for the species were determined at control (i.e. non-stress) conditions. For HSP70, the base levels were positively correlated to the species’ thermotolerances. For sHSP, however, only thermotolerant G. fasciatus showed a high level.Thermalstressat 25 C water temperature caused a deferred onset of HSP70 and sHSP expression followed by a subsequent offset, delineating a unimodal response curve. The time lag to the expression on set of HSP70 was shorter in the thermosensitive species, compared to thermotolerant ones. Conversely, the time span until the maximum level of HSP70 was variable, not showing a dependence on the thermotolerance properties of the species. The peak concentration in G. pulex was distinctly higher than in the other species, whereas E. verrucosus did not develop a well-defined response maximum at all. In sHSP,the temporal pattern of expression was even more variable than in HSP70. However,the thermosensitive species E. verrucosus showed a time lag of expression onset significantly shorter than the other species and thermotolerant G. fasciatus developed the most pronounced response maximum. Basing on these results, the cellular response to thermalstress in amphipods is more consistently reflected by HSP70,compared to sHSP.
Bibliographical description: Shatilina Z.M., H.W.Riss, M.V.Protopopova, M.Trippe, E.I.Meyer, V.V.Pavlichenko, D.S.Bedulina, D.V. Axenov-Gribanov, M.A. Timofeyev.The role of heat the shock proteins (HSP70 and sHSP) for the thermotolerance of freshwater amphipods from contrasting habitats. Journal of thermal biology, 2011, V.36, I.2, P. 142 – 149.
Publication's type: статья
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